Histological and Clinical Responses of Articular Cartilage to Low-level Laser Therapy: Experimental Study

 

I. RUIZ CALATRAVA, J.M. SANTISTEBAN VALENZUELA, R.J. GOMEZ-VILLAMANDOS, J.I. REDONDO, J.C. GOMEZ-VILLAMANDOS, 1. AVIGA JURADO

 

Department of Veterinary Clinical Pathology-Surgery, Faculty of Veterinary Medicine, University of Cordoba, Spain.

Correspondence to 1. Ruiz Clatrava, Department of Veterinary Clinical Pathology-Surgery, Faculty of Veterinary Medicine, University of Cordoba, Avda, Medina Azahara, 9, 14005 Cordaba Spain

 

Abstract. This study was carried out to evaluate the effects of low-level laser irradiation on experimental lesions of articular cartilage. A standard lesion was practiced on the femoral trochlea of both hind limbs of 20 clinically normal Californian rabbits. These animals were divided into two groups of 10 individuals each, depending on the laser equipment used for treatment. One group was treated with HeNe laser (8J/cm2-2, 632.8nm wavelength) and the other with infra-red (JR) laser (8J/cm2-2, 904nm wavelength). In both groups, five points of irradiation to the right limb alone were irradiated per session for a total of 13 sessions. These points were the following: left and right femoral epicondyles, left and right tibial condyles and the centre of articulation. The distance between these points was approximately 1cm. The untreated left limb was left as a control. During treatment, extension angle and treatment, samples were collected for histopathological study and stained with: Haematoxylin-Eosin, PAS and Done. The results show a statistically higher anti-inflammatory capacity of the IR laser (9<0.0001). The functional recovery was statistically similar for both treatments (p<0. 176). Histological study showed, at the end of the treatment, hyaline cartilage in the IR group, fibrocartilage in the HeNe group and granulation tissue in the control limbs. Clinical and histological results indicated that this laser treatment had a clear anti-inflammatory effect that provided a fast recuperation and regeneration of the articular cartilage.